bio-splicing-qc
bio-splicing-qc is a Bioinformatics & life sciences skill for AI agents, published by GPTomics in the bioSkills repository on GitHub. Skillspace rates bio-splicing-qc F (45/100) and the safety scan rated it risky; the rating is based on static analysis only.
Risky. This skill has patterns associated with unsafe behaviour. Read the source carefully before running it.
Assesses RNA-seq data quality specifically for alternative splicing analysis. QC layers include experimental design audit (library prep, read length, depth, replicates), STAR 2-pass cohort-style alignment, junction saturation curves and discovery plateau detection, novel-vs-known junction ratio diagnostics, junction-overhang distribution, splice-site strength scoring (MaxEntScan intrinsic + SpliceAI context-aware), strandedness verification, GENCODE basic vs comprehensive choice, and rRNA contamination screening. Splicing analysis is more demanding than DGE on read length, depth, library prep, alignment strategy, and annotation choice — failures silently bias PSI estimates and inflate novel-junction false positives. Use when evaluating data suitability for splicing analysis, troubleshooting low event detection, or designing sequencing experiments where AS is a primary endpoint.
- Grade
- F
- Quality
- 45 / 100
- Safety
- Risky
- GitHub stars
- 1,202
Unproven: rated by static analysis only, not yet compared head-to-head.
This rating describes the version of the repository we crawled; it may have changed since.
Our review
- Architecture
- 93 / 100
- Maturity
- Basic: a single SKILL.md
- Security scan
- High-risk patterns
- License
- Not declared. Check the repository before reusing it.
What the scanner flagged
- executes shell/subprocess
Not checked yet
- Category is a classifier prediction; no model has read this skill yet
- Not judged on the rubric yet (not a contender in its category)
- No blind task eval yet
- Scope (portable / vendor / repo-internal) not assessed; only the top of each category is read for scope
- Sibling files (scripts, references) not fetched; security scan covers SKILL.md only
Also published in 1 other repo
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